Barcelona were denied the chance to open up a three-point lead at the top of La Liga after Valencia won 2-0.
Jordi Alba scored an own goal, while Maxi Gomez also found the net for Valencia at the Mestalla to slow down Barca's momentum in the title race.
It's a first defeat for Quique Setien since he replaced Ernesto Valverde in the Barcelona dugout.
He won his first game 1-0 but then almost stumbled in the cup against a third tier side and saw Barcelona's terrible defensive record continue here.
There was a lot of optimism and positivity after Setièn’s first match in charge last weekend, but the honeymoon period will well and truly be over after this showing.