Seven people were killed when the bus they were travelling in overturned on the R532 between Graskop and Ohrigstad on Sunday.
The bus they were travelling in overturned on the R532 Highway.
Department of Community Safety in Mpumalanga spokesperson Moeti Mmusi said all seven died at the scene.
"About 39 of those who were injured are currently receiving treatment at Matibidi and Kiaat hospitals. The deceased are all males."
Mmusi added: "The MEC for community and security is sending condolences to the affected families and is wishing those who have been injured a speedy recovery. The MEC further calls on motorists to use roads with caution."
Provincial police are investigating the cause of the crash.